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article 2016 16 pages

Validity and Reliability of Ventilatory and Blood Lactate Thresholds in Well-Trained Cyclists

Jesús G. Pallares, Ricardo Morán-Navarro, Juan Fernando Ortega, Valentín Emilio Fernández-Elías, Ricardo Mora-Rodríguez

Journal
PLOS ONE
DOI
10.1371/journal.pone.0163389
Publication type
Research Article
Study type
experimental study
Population
aerobically-trained male cyclists
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Abstract

Purpose

The purpose of this study was to determine, i) the reliability of blood lactate and ventilatory-based thresholds, ii) the lactate threshold that corresponds with each ventilatory threshold (VT1 and VT2) and with maximal lactate steady state test (MLSS) as a proxy of cycling performance.

Methods

Fourteen aerobically-trained male cyclists ( 62.1±4.6 ml·kg-1·min-1) performed two graded exercise tests (50 W warm-up followed by 25 W·min-1) to exhaustion. Blood lactate, and data were collected at every stage. Workloads at VT1 (rise in ;) and VT2 (rise in ) were compared with workloads at lactate thresholds. Several continuous tests were needed to detect the MLSS workload. Agreement and differences among tests were assessed with ANOVA, ICC and Bland-Altman. Reliability of each test was evaluated using ICC, CV and Bland-Altman plots.

Results

Workloads at lactate threshold (LT) and LT+2.0 mMol·L-1 matched the ones for VT1 and VT2, respectively (p = 0.147 and 0.539; r = 0.72 and 0.80; Bias = -13.6 and 2.8, respectively). Furthermore, workload at LT+0.5 mMol·L-1 coincided with MLSS workload (p = 0.449; r = 0.78; Bias = -4.5). Lactate threshold tests had high reliability (CV = 3.4–3.7%; r = 0.85–0.89; Bias = -2.1–3.0) except for DMAX method (CV = 10.3%; r = 0.57; Bias = 15.4). Ventilatory thresholds show high reliability (CV = 1.6%–3.5%; r = 0.90–0.96; Bias = -1.8–2.9) except for RER = 1 and V-Slope (CV = 5.0–6.4%; r = 0.79; Bias = -5.6–12.4).

Conclusions

Lactate threshold tests can be a valid and reliable alternative to ventilatory thresholds to identify the workloads at the transition from aerobic to anaerobic metabolism.

Citation: Pallarés JG, Morán-Navarro R, Ortega JF, Fernández-Elías VE, Mora-Rodriguez R (2016) Validity and Reliability of Ventilatory and Blood Lactate Thresholds in Well-Trained Cyclists. PLoS ONE 11(9):
e0163389.

https://doi.org/10.1371/journal.pone.0163389

Editor: Øyvind Sandbakk, Norwegian University of Science and Technology, NORWAY

Received: April 26, 2016; Accepted: September 6, 2016; Published: September 22, 2016

Copyright: © 2016 Pallarés et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: The authors received no specific funding for this work.

Competing interests: The authors have declared that no competing interests exist.

Description

This study evaluates the reliability of lactate and ventilatory thresholds in cycling performance.